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rotary magnetic tumble stirrer  (V&P Scientific)


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    Structured Review

    V&P Scientific rotary magnetic tumble stirrer
    Rotary Magnetic Tumble Stirrer, supplied by V&P Scientific, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rotary+magnetic+tumble+stirrer/VP+772DP-N42-5-2/pm41240912-381-13-17
    Average 94 stars, based on 7 article reviews
    rotary magnetic tumble stirrer - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Lysis:

    Article Title: Brain and blood single-cell transcriptomics in acute and subacute phases after experimental stroke
    Article Snippet: Barcoded capture beads (ChemGenes Corporation, Wilmington, MA) were resuspended in 1.8 ml lysis buffer consisting of 4M Guanidine HCL (Thermofisher Scientific, Waltham, MA), 6% Ficoll PM-400 (Sigma-Aldrich), 0.2% Sarkosyl (Sigma-Aldrich, St. Louis, MO), 20mM EDTA (Thermofisher Scientific), 200mM Tris pH 7.5 (Sigma-Aldrich), 50mM DTT (Sigma-Aldrich) at a concentration of 120 beads/μl. .. A 5mm diameter, 1.7mm thick PVDF encapsulated magnetic stir disc and rotary magnetic tumble stirrer (V&P Scientific, San Diego, CA) was used along with a 3 ml syringe that contained the beads in lysis buffer to keep the beads in suspension. .. Thereafter, single cells and beads were encapsulated in nanoliter-scale droplets using a Drop-seq microfluidic device coated with Aquapel (FlowJEM, Toronto Canada), droplet generation oil (BioRad, Hercules, CA) using flow rates of 4 ml/h for cells and beads and 15 ml/h for oil.

    Article Title: Hypertension-induced neurovascular and cognitive dysfunction at single-cell resolution.
    Article Snippet: In brief Hypertension is a major cause of cognitive decline, but the underlying mechanisms are unclear.. Using scRNAseq in an angiotensin II mouse model, Schaeffer and Pacholko identify early endothelial, interneuron, and oligodendrocyte vulnerability linked to angiotensin II signaling, revealing molecular pathways that drive later neurovascular dysfunction and cognitive impairment in hypertension.

    Article Title: Hypertension-Induced Neurovascular and Cognitive Dysfunction at Single-Cell Resolution
    Article Snippet: Barcoded capture beads (ChemGenes Corporation, Wilmington, MA) were resuspended in 1.8 ml lysis buffer consisting of 4M Guanidine HCL (Thermofisher Scientific, 24110), 6% Ficoll PM-400 (Sigma-Aldrich, F4375), 0.2% Sarkosyl (Sigma-Aldrich, 61747), 20mM EDTA (Thermofisher Scientific, 17892), 200mM Tris pH 7.5 (Sigma-Aldrich, DB0339), and 50mM DTT (Sigma-Aldrich, D0632) at a concentration of 120 beads/μl. .. A 5 mm diameter, 1.7 mm thick PVDF encapsulated magnetic stir disc and rotary magnetic tumble stirrer (V&P Scientific, VP772DP-N42-5-2) was used along with a 3 ml syringe that contained the beads in lysis buffer to maintain the beads in suspension. .. Next, single cells and beads were encapsulated in nanoliter-scale droplets using a Drop-seq microfluidic device coated with Aquapel (FlowJEM); flow rates were 15 ml/hour for the Droplet generation oil (BioRad, 1863005) and 4 ml/hour for the cells and beads.

    Article Title: Brain and blood single-cell transcriptomics in acute and subacute phases after experimental stroke
    Article Snippet: Barcoded capture beads (ChemGenes Corporation, Wilmington, MA) were resuspended in 1.8 ml lysis buffer consisting of 4M Guanidine HCL (Thermofisher Scientific, Waltham, MA), 6% Ficoll PM-400 (Sigma-Aldrich), 0.2% Sarkosyl (Sigma-Aldrich, St. Louis, MO), 20mM EDTA (Thermofisher Scientific), 200mM Tris pH 7.5 (Sigma-Aldrich), 50mM DTT (Sigma-Aldrich) at a concentration of 120 beads/μl. .. A 5mm diameter, 1.7mm thick PVDF encapsulated magnetic stir disc and rotary magnetic tumble stirrer (V&P Scientific, San Diego, CA) was used along with a 3 ml syringe that contained the beads in lysis buffer to keep the beads in suspension. .. Thereafter, single cells and beads were encapsulated in nanoliter-scale droplets using a Drop-seq microfluidic device coated with Aquapel (FlowJEM, Toronto Canada), droplet generation oil (BioRad, Hercules, CA) using flow rates of 4 ml/h for cells and beads and 15 ml/h for oil.

    Suspension:

    Article Title: Brain and blood single-cell transcriptomics in acute and subacute phases after experimental stroke
    Article Snippet: Barcoded capture beads (ChemGenes Corporation, Wilmington, MA) were resuspended in 1.8 ml lysis buffer consisting of 4M Guanidine HCL (Thermofisher Scientific, Waltham, MA), 6% Ficoll PM-400 (Sigma-Aldrich), 0.2% Sarkosyl (Sigma-Aldrich, St. Louis, MO), 20mM EDTA (Thermofisher Scientific), 200mM Tris pH 7.5 (Sigma-Aldrich), 50mM DTT (Sigma-Aldrich) at a concentration of 120 beads/μl. .. A 5mm diameter, 1.7mm thick PVDF encapsulated magnetic stir disc and rotary magnetic tumble stirrer (V&P Scientific, San Diego, CA) was used along with a 3 ml syringe that contained the beads in lysis buffer to keep the beads in suspension. .. Thereafter, single cells and beads were encapsulated in nanoliter-scale droplets using a Drop-seq microfluidic device coated with Aquapel (FlowJEM, Toronto Canada), droplet generation oil (BioRad, Hercules, CA) using flow rates of 4 ml/h for cells and beads and 15 ml/h for oil.

    Article Title: Hypertension-induced neurovascular and cognitive dysfunction at single-cell resolution.
    Article Snippet: In brief Hypertension is a major cause of cognitive decline, but the underlying mechanisms are unclear.. Using scRNAseq in an angiotensin II mouse model, Schaeffer and Pacholko identify early endothelial, interneuron, and oligodendrocyte vulnerability linked to angiotensin II signaling, revealing molecular pathways that drive later neurovascular dysfunction and cognitive impairment in hypertension.

    Article Title: Hypertension-Induced Neurovascular and Cognitive Dysfunction at Single-Cell Resolution
    Article Snippet: Barcoded capture beads (ChemGenes Corporation, Wilmington, MA) were resuspended in 1.8 ml lysis buffer consisting of 4M Guanidine HCL (Thermofisher Scientific, 24110), 6% Ficoll PM-400 (Sigma-Aldrich, F4375), 0.2% Sarkosyl (Sigma-Aldrich, 61747), 20mM EDTA (Thermofisher Scientific, 17892), 200mM Tris pH 7.5 (Sigma-Aldrich, DB0339), and 50mM DTT (Sigma-Aldrich, D0632) at a concentration of 120 beads/μl. .. A 5 mm diameter, 1.7 mm thick PVDF encapsulated magnetic stir disc and rotary magnetic tumble stirrer (V&P Scientific, VP772DP-N42-5-2) was used along with a 3 ml syringe that contained the beads in lysis buffer to maintain the beads in suspension. .. Next, single cells and beads were encapsulated in nanoliter-scale droplets using a Drop-seq microfluidic device coated with Aquapel (FlowJEM); flow rates were 15 ml/hour for the Droplet generation oil (BioRad, 1863005) and 4 ml/hour for the cells and beads.

    Article Title: Brain and blood single-cell transcriptomics in acute and subacute phases after experimental stroke
    Article Snippet: Barcoded capture beads (ChemGenes Corporation, Wilmington, MA) were resuspended in 1.8 ml lysis buffer consisting of 4M Guanidine HCL (Thermofisher Scientific, Waltham, MA), 6% Ficoll PM-400 (Sigma-Aldrich), 0.2% Sarkosyl (Sigma-Aldrich, St. Louis, MO), 20mM EDTA (Thermofisher Scientific), 200mM Tris pH 7.5 (Sigma-Aldrich), 50mM DTT (Sigma-Aldrich) at a concentration of 120 beads/μl. .. A 5mm diameter, 1.7mm thick PVDF encapsulated magnetic stir disc and rotary magnetic tumble stirrer (V&P Scientific, San Diego, CA) was used along with a 3 ml syringe that contained the beads in lysis buffer to keep the beads in suspension. .. Thereafter, single cells and beads were encapsulated in nanoliter-scale droplets using a Drop-seq microfluidic device coated with Aquapel (FlowJEM, Toronto Canada), droplet generation oil (BioRad, Hercules, CA) using flow rates of 4 ml/h for cells and beads and 15 ml/h for oil.



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